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. 2018 Apr 12;61(9):4135–4154. doi: 10.1021/acs.jmedchem.8b00210

Figure 8.

Figure 8

Compound 1 in combination with VEGFA reduces A375P spheroid outgrowth. (A) A375P cells were resuspended and used to generate spheroids as described in Materials and Methods. Then 24 h after spheroid production, spheroids were imbedded in a collagen gel and incubated in medium containing 0.1% DMSO as a vehicle control (Veh), VEGF 25 ng/mL, 1 (30 μM), and 1 (30 μM) + VEGF 25 ng/mL for 7 days. (B) Spheroid outgrowth area was measured by quantifying the area corresponding to the invasion rim minus the area of the core for at least four different spheroids per condition. Data from at least three independent experiments ± SEM are presented as arbitrary units (a.u.) *p < 0.05.