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. Author manuscript; available in PMC: 2018 May 17.
Published in final edited form as: Immunity. 2017 Mar 14;46(3):446–456. doi: 10.1016/j.immuni.2017.02.012

Figure 2. 25HC Broadly Inhibits Flaviviruses Infection.

Figure 2

(A and B) Vero cells were pretreated with EtOH or indicated dose of 25HC for 8 hr, followed by ZIKV infection (GZ01/2016 strain, MOI 0.1). The titer of ZIKV in the supernatant was quantified by (A) plaque assay and (B) qRT-PCR.

(C–F) Vero cells were pretreated with indicated amount of 25HC for 8 hr and then infected with (C) ZIKV GZ01/2016 strain, (D) DENV, (E) YFV, and (F) WNV at MOI 0.1. Viral RNA copies in cell lysates were quantified by qRT-PCR. IC50 of 25HC to ZIKV, DENV, YFV, and WNV were calculated and indicated. All data are shown as mean ± SEM from three independent experiments. **p ≤ 0.01, unpaired Student’s t test. See also Figure S4.

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