Figure 5. 25HC Protects Rhesus Monkeys from ZIKV Infection.
(A and B) Rhesus monkeys were injected i.v. with 25HC (1.5 mg/kg) (n = 2) or the vehicle control EtOH (n = 3), followed by infection with 1×103 PFU of ZIKV (GZ01/2016 strain) i.m. Animals were treated with 25HC or EtOH daily for 7 days. Viral copy numbers in (A) serum and (B) urine of control monkeys or 25HC-pretreated were measured by qRT-PCR.
(C) The level of IFN-γ, IL-15, and CXCL9 in the serum of infected animals was measured at 0, 3, and 7 dpi by Luminex assay. Data of (C) are shown as mean ± SD from three experimental replicates, *p ≤ 0.05, **p ≤ 0.01, unpaired Student’s t test.
See also Figure S6.