Table 4.
Sheath and spermathecal markers are expressed in animals that show evidence of ovulation defects
Marker | L1 RNAia | % Oocyte piecesb | % expressing marker | nc | 1-sided Fisher exact |
---|---|---|---|---|---|
lim-7::mCherry | Controld | 0% | 100% | 42 | |
lim-7::mCherry | apx-1 | 21% | 100% | 155 | *** |
lim-7::mCherry | lin-12 | 10% | 100% | 125 | * |
let-502::GFP | Controld | 0% | 100% | 30 | |
let-502::GFP | apx-1 | 21% | 100% | 24 | * |
let-502::GFP | lin-12 | 13% | 100% | 30 | ns |
Due to variability of RNAi, results are separated into parallel control experiments.
Fragments of oocyte in oviduct, spermatheca or uterus as scored with DIC
n = number of gonad arms
Control RNAi is the L4440 “empty vector” plasmid in E. coli HT115.
Statistics: 1-sided Fisher exact test, ns= p>0.05, * = p<0.05, ***= p<0.001, in pairwise comparison with control RNAi in the same marker strain.