(A and B) PAM specificities of the RVR (A) and RR (B) variants. The AsCpf1-crRNA complex (100 nM) was incubated at 37°C for 5 min with a linearized plasmid target with the different PAMs. The favorable PAMs for the RVR (TATA) and RR (TCCC) variants are boxed in red. The substituted nucleotides are colored red.
(C) Fourth PAM nucleotide preferences of WT AsCpf1 and the RVR and RR variants. The AsCpf1-crRNA complex (100 nM) was incubated at 37°C for 5 min with a linearized plasmid target with the TTTN PAMs. The substituted nucleotides are colored red.
See also Figures S1 and S2.