Table 1.
Data collection and refinement statistics.
| RVR (TATA PAM) | RR (TCCA PAM) | |
|---|---|---|
| Data collection | ||
| Beamline | SPring-8 BL41XU | SPring-8 BL41XU |
| Wavelength (Å) | 1.0000 | 1.0000 |
| Space group | P212121 | P212121 |
| Cell dimensions | ||
| a, b, c (Å) | 81.2, 133.7, 199.6 | 80.7, 133.3, 200.0 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å)* | 40.0–2.0 (2.03–2.00) | 40.0–2.0 (2.03–2.00) |
| Rpim | 0.021 (0.396) | 0.034 (0.466) |
| I/σI | 17.1 (2.0) | 10.5 (1.5) |
| Completeness (%) | 98.2 (98.3) | 99.9 (99.9) |
| Multiplicity | 6.6 (6.6) | 6.4 (6.4) |
| CC(1/2) | 0.999 (0.813) | 0.999 (0.841) |
| Refinement | ||
| Resolution (Å) | 40.0–2.0 (2.02–2.00) |
40.0–2.0 (2.02–2.00) |
| No. reflections | 143,851 (4,771) | 145,320 (4,787) |
| Rwork/Rfree | 0.175/0.210 (0.272/0.333) |
0.183/0.214 (0.256/0.304) |
| No. atoms | ||
| Protein | 10,459 | 10,431 |
| Nucleic acid | 1,639 | 1,639 |
| Ion | 6 | 6 |
| Solvent | 731 | 747 |
| B-factors (Å2) | ||
| Protein | 51.8 | 49.5 |
| Nucleic acid | 50.2 | 48.2 |
| Ion | 50.1 | 45.2 |
| Solvent | 52.3 | 49.6 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.007 | 0.006 |
| Bond angles (°) | 0.843 | 0.834 |
| Ramachandran plot (%) | ||
| Favored region | 98.27 | 98.12 |
| Allowed region | 1.65 | 1.80 |
| Outlier region | 0.08 | 0.08 |
| MolProbity score | ||
| Clashscore | 2.68 | 2.48 |
| Rotamer outlier | 2.20 | 2.30 |
*
Values in parentheses are for the highest resolution shell.