Table 1.
Primary Antibody Table
Antibody | Immunogen | Manufacturer, Calalog #, RRID, Host Species, Monoclonal/Polyclonal | Concentration Used |
---|---|---|---|
1. Cocaine- and amphetamine-regulated transcript(CART), 55–102 | Ile - Pro - Ile - Tyr - Glu - Lys - Lys - Tyr - Gly - Gln - Val - Pro - Met - Cys - Asp - Ala - Gly - Glu - Gln - Cys - Ala - Val - Arg - Lys - Gly - Ala - Arg - Ile - Gly - Lys - Leu - Cys - Asp - Cys - Pro - Arg - Gly - Thr - Ser - Cys - Asn - Ser - Phe - Leu - Leu - Lys - Cys - Leu [Disulfide bonds between Cys 1 - Cys 3, Cys 2 - Cys 5, Cys 4 - Cys 6] | Phoenix Pharmaceuticals, H-003-62, RRID:AB_2313614, Rabbit, Polyclonal | 1:10,000 |
2. Choline Acetyltransferase (ChAT) | Purified human placental ChAT enzyme | Millipore, AB144P, RRID:AB_2079751, Goat, Polyclonal | 1:500 |
3. Green Fluorescent Protein (GFP) | Recombinant full length protein corresponding to GFP | Abcam, ab13970, RRID:AB_300798, Chicken, Polyclonal | 1:500 |
4. Green Fluorescent Protein (GFP) | GFP emulsified in Freund’s adjuvant | Aves Labs, GFP-1020, RRID:AB_10000240, Chicken, Polyclonal | 1:400 |
5. MET receptor tyrosine kinase (MET), Clone 25H2 | Synthetic peptide containing amino acid residues 1228–1243 of human Met c-terminus | Cell Signaling Technology, 3127, RRID:AB_331361, Mouse, Monoclonal | 1:400 |
6. MET receptor tyrosine kinase (MET/HGF-R) | Recombinant mouse Met, Glu25-Asn929 | R and D Systems, AF527, RRID:AB_355414, goat, Polyclonal | 1:500 |
7. Neurofilament | E14–E15 rat spinal cord | Developmental Studies Hybridoma Bank (DSHB), 2H3-c, RRID:AB_531793, Mouse, Monoclonal | 1:1,000 for tissue sections, 1:500 for whole mount |
8. Tyrosine Hydroxylase (TH) | Sodium dodecyl sulfate denatured tyrosine hydroxylase from rat pheochromocytoma | Millipore, AB152, RRID:AB_390204, Rabbit, Polyclonal | 1:1,000 |