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. Author manuscript; available in PMC: 2018 May 17.
Published in final edited form as: J Phys Chem Lett. 2017 Jul 25;8(15):3642–3647. doi: 10.1021/acs.jpclett.7b01509

Figure 3.

Figure 3

A) The average step size of unfolding and folding traces as a function of force, fit with the FJC model of Kh = 1.1 nm and ΔLc = 16.3 nm. Error bars represent standard deviation. The insert is a zoom of the low-forces regime. B) Measurements of the probability of folding (red) and the most likely state (Global minimum, GM; blue) as a function of refolding force obtained from traces similar to those in Figure 1A. The free-energy model with a Morse potential of UM0= 1.8 kT provides the force dependency of the global minimum (most likely state) that fits the experimental data (solid black line). C) Unfolding rates vs force from unfolding traces at a pulling force (13 – 120 pN) using AFM (blue symbols), and MT (red symbols). Brownian dynamics (BD) simulations of unfolding traces were run on our free-energy model (green curve) with UG0 = 12 kT, σ = 0.025 nm, and x‡ = 0.44 nm. Error bars represent standard errors of the mean.