Table 2. Primer sequences.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| Reference genes | |||
|
Hprt |
ATGGGAGGCCATCACATTGT |
ATGTAATCCAGCAGGTCAGCAA |
77 |
|
Ywhaz |
CAAGCATACCAAGAAGCATTTGA |
GGGCCAGACCCAGTCTGA |
76 |
|
Rho |
GCAACAGGAGTCGGCTACCA |
GCATAGGGAAGCCAGCAGATC |
76 |
| Target genes | |||
|
Tnf |
CCCAATCTGTGTCCTTCT |
TTCTGAGCATCGTAGTTGT |
90 |
|
Il-1β |
ATAGAAGTCAAGACCAAAGTG |
GACCATTGCTGTTTCCTAG |
109 |
|
NOS2 |
AGAGACAGAAGTGCGATC |
AGATTCAGTAGTCCACAATAGTA |
96 |
|
Pou4fi |
ACCCTGTTCGTTTCAAATAG |
AGCGTTTATCCCTGGTAAT |
104 |
|
Tspo |
TGTATTCGGCCATGGGGTATG |
GAGCCAGCTGACCAGTGTAG |
105 |
| Qualitative PCR | |||
| Actb | GCTCCTCCTGAGCGCAAG | CATCTGCTGGAAGGTGGACA | 75 |