Extended Data Table 2.
Binding and behavioral activity summary for DISSECTIV results.
| Measurement | KLH | FEN-KLH | THEO-KLH | 1-A1-KLH |
|---|---|---|---|---|
| Fenethylline pIC50 | >3.00 | 5.912 ± 0.079 | 5.727 ± 0.021 | 5.000 ± 0.022 |
| Theophylline pIC50 | >3.00 | 5.378 ± 0.079 | 5.385 ± 0.022 | >3.00 |
| Amphetamine pIC50 | >3.00 | >3.00* | >3.00 | 4.707 ± 0.012 |
| Methamphetamine pIC50 | >3.00 | >3.00* | >3.00 | 3.682 ± 0.031 |
| Cocaine pIC50 | >3.00 | >3.00 | >3.00 | >3.00 |
| FEN-BSA Binding(OD) | 0.076 ± 0.013 | 0.539 ± 0.112 | 0.321 ± 0.107 | 0.064 ± 0.054* |
| THEO-BSA Binding(OD) | 0.066 ± 0.003 | 0.372 ± 0.033 | 1.226 ± 0.192 | 0.062 ± 0.056 |
| 1-A1-BSA Binding(OD) | 0.070 ±0.051 | 0.306 ± 0.005 | 0.071 ± 0.067 | 2.590 ± 0.023 |
| Serum Fenethylline (μM)† | 15.76 ±6.21 | 43.40 ±10.90 | 36.70 ±3.81 | 33.53 ±8.25 |
| Serum Theophylline (μM)† | 90.80 ±12.68 | 127.20±22.10 | 151.20±16.87 | 51.50 ±12.12 |
| Serum Amphetamine (μM)† | 18.79 ±2.51 | 30.07 ±4.26 | 20.40 ±1.08 | 31.73 ±6.18 |
| Locomotor Activity‡ | 326.1 ±62.88 | 95.86 ± 24.73 | 270.70± 73.89 | 128.30± 27.64 |
| Plus Maze Activity‡ | 51.31 ±17.32 | 108.80± 22.99 | 62.46 ± 19.00 | 106.20± 16.17 |
| Place Preference Activity‡ | 100.0±22.60 | 53.60 ± 48.29 | 57.79± 31.84 | 62.00± 44.84 |
*
Binding verified in alternative assay.
†
Concentration measurements taken at 15 minutes post-injection.
‡
Percent of baseline (20 mg/kg fenethylline). Data shown as mean ± SEM.