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. 2018 May 17;12(4):042205. doi: 10.1063/1.5022879

FIG. 2.

FIG. 2.

Cancer cell adhesion on inflamed endothelial cells under dynamic conditions. (a) Representative fluorescence microscopy images of breast cancer cells MDA-MB-231 (cell membrane labeled in red with CM-DIL) flowing and interacting, in a single-channel microfluidic chip, with a confluent monolayer of HUVECs (cell nuclei stained in blue with DAPI). (b) Normalized number of adhering cancer cells on a HUVEC monolayer at a flow rate of 50 nl/min, with and without stimulation with TNF-α (25 ng/ml), for 6 and 12 h. (c) Representative fluorescence microscopy images of colon cancer cells HCT-15 (cell membrane labeled in red with CM-DIL) flowing and interacting in a single-channel microfluidic chip, with a confluent monolayer of HUVECs (cell nuclei stained in blue with DAPI). (d) Normalized number of adhering cancer cells on a HUVEC monolayer at a flow rate of 100 nl/min, with and without stimulation with TNF-α (25 ng/ml), for 6 and 12 h [Data are plotted as mean ± SD. n = 3. Statistical analysis ANOVA: *** symbol denotes statistically significant difference p < 0.0001; ** symbol denotes statistically significant difference p < 0.001. (ninj = 106 cells and A = 1.22 × 10−6 m2). HUVECs are not stimulated with TNF-α (-TNF-α) or stimulated with 25 ng/ml TNF-α for 6h (+TNF-α 6 h) or 12h (+TNF-α 12 h)].