Fig. 3.

qPCR analysis of caveolin and clathrin genes regulating the process of endocytosis of nano-lipoplexes. A) Schematic illustrating the role of main proteins regulating the caveolin-mediated endocytosis. This process is divided into three steps: (1) Dynamic of caveolae at the plasma membrane, (2) Caveolae internalization by actin fibers stress and (3) endocytotic trafficking by microtubules. B) Fold increase expression of the main genes involved in caveolin endocytosis pathways after 1 and 4 hours of lipoplexes internalization. C) Fold increase expression of the main genes regulating clathrin endocytosis pathways after 1 and 4 hours of lipoplexes internalization. Results are normalized based on the gene expression of hASCs cultured on the soft substrate (0.5 kPa) (n=3). *= p < 0.05, ** = p < 0.01.