Skip to main content
. Author manuscript; available in PMC: 2018 Aug 26.
Published in final edited form as: Oncogene. 2018 Feb 26;37(20):2615–2629. doi: 10.1038/s41388-018-0138-y

Figure 5. UGDH Knockdown decreases GBM cell proliferation and clonogenicity.

Figure 5

(A) Cell growth curve up to 9 days after plating. Trypsinized cells were stained with Trypan blue and both viable (unlabeled) cells were counted on the days indicated. (B) Colony formation assays showing significant decrease in anchorage independent clongenicity in UGDH knockdown U87 cells (right panel). For GBM neurosphere cells, equal numbers of viable GBM1A cells were plated and cultured for 14 days to allow neurosphere formation. Neurospheres (>100 μm diameter) were counted with MCID software. UGDH silencing inhibited neurosphere formation. (C) Cell cycle was synchronized. There was a delayed progression to S phase in U87 UGDH knockdown cells compared to controls after 32 hrs of serum replenishing. (D) UGDH silencing decreased cyclin D1 and E protein levels in GBM cells. (E). HA (75ug/ml) supplemented in U87 cells rescued the decreased cell growth effect observed in UGDH knockdown cells on days 6 and 9. (F) Phosphorylated AKT levels were significantly decreased in UGDH knockdown U87 cells compared to controls as evidenced by Western blots (*: P < 0.05; ** P<0.01; *** P<0.001).