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. Author manuscript; available in PMC: 2018 Oct 8.
Published in final edited form as: J Allergy Clin Immunol. 2017 Nov 17;142(4):1159–1172.e5. doi: 10.1016/j.jaci.2017.08.046

Figure 2. IL-4 amplifies histamine-induced vascular endothelial barrier dysfunction.

Figure 2

Percent reduction in transendothelial resistance (TER) (A) and horseradish peroxidase (HRP) flux (B) of vehicle- or IL-4-treated EA.hy926 cells following histamine stimulation. EA.hy926 monolayers were pretreated with vehicle or IL-4 (100 ng/mL) for 24 hrs and stimulated with histamine (100 μM for 30 min) or vehicle and TER and HRP flux were measured as described in methods. Data are represented as the mean ± SD; n = 3 wells per group from n = 2 independent experiments (A, B). **** P < 0.0001, *** P < 0.0001, ** P < 0.01, ns > 0.05.