Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 17;9:1974. doi: 10.1038/s41467-018-04353-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Hypotonicity-induced depolarization of β-cells. Gramicidin-perforated patch-clamp recordings of primary mouse β-cells. a Resting membrane potential of control Lrrc8a^lox/lox or βc-Δ8a β-cells at 1 or 5 mM glucose concentrations. Mean values ± SEM indicated by error bars. b Representative trace for hypotonicity-induced depolarization of the membrane potential V_m in control Lrrc8a^lox/lox animals at 5 mM glucose. TOLB, tolbutamide. c ΔV_m after applying hypotonicity, or following washout with isotonic solution of Lrrc8a^lox/lox or βc-Δ8a cells at 5 mM glucose, calculated from data as indicated in b. Spiking cells (see g and h) were excluded from analysis. d Representative voltage trace showing lack of hypotonicity-induced depolarization of βc-Δ8a β-cells at 5 mM glucose. e ΔV_m of Lrrc8a^lox/lox or βc-Δ8a cells as in c, but at 1 mM glucose. f Representative trace for Lrrc8a^lox/lox β-cells challenged with hypotonicity in the presence of 1 mM glucose. g Percentage of cells responding with electrical activation following hypotonic stimulation. h Voltage trace of an Lrrc8a^lox/lox β-cell responding with electrical activity to hypotonicity in the presence of 5 mM glucose. *p < 0.05, **p < 0.01 (one-way ANOVA, Tukey’s test)