Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 17;9:1981. doi: 10.1038/s41467-018-04366-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — System L flow cytometry uptake assay with a mixed cell population. a Frequency of CD8⁺ T cells, b CD44 and c IFNγ expression in CD8⁺ T cells from spleen 7 days after recombinant Listeria monocytogenes (rLM) infection compared with uninfected controls (UIC). d 450 nm fluorescence emission of CD8⁺ T cells from uninfected controls (UIC; left) or D7 rLM (right) treated with kynurenine in the presence or absence of BCH (10 mM), Leu (5 mM) or Lys (5 mM) at (37 °C, 4 min). e Ratio of the 450 nm emission of CD8⁺ T cells treated with kynurenine ± BCH 10 mM (4 min, 37 °C). f, g Mean 450 nm fluorescence emission of CD8⁺ T cells from UIC, D7 rLM or CTL treated with kynurenine in the presence of increasing concentrations of BCH (f) or leucine (g) at (37 °C, 4 min). h CD98 surface expression of CD8⁺ T cells from UIC, D7 rLM or CTL. Error bars are ±s.d. from five biological replicates. Points indicate biological replicates (a–c, e). MFIs are indicated in the histograms (d), data are representative of five biological replicates (d, f–h). P values * = < 0.01; ** = < 0.005; *** = < 0.001; **** = < 0.0001 (unpaired t-test)