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. 2018 May 17;8:7796. doi: 10.1038/s41598-018-26078-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pupil metrics in response to pulsed and sinusoidal stimulation. The minimum amplitude (Panel A), PIPR amplitude (Panel B), Peak to trough amplitude (Panel C) and phase amplitude percentage (Panel D) are depicted. Data in Panel A are derived from pulsed stimulation and data in Panels C and D are derived from sinusoidal stimulation. Each data point represents an individual’s mean data, boxplots depict the quartiles and whiskers the range. Asterisks indicate a significant difference between groups (p < 0.05).