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. 2018 May 17;8:7787. doi: 10.1038/s41598-018-26194-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence location and quantification of CCL20 and GRO by confocal microscopy in the colonic sections of mice used in the experiments. C (uninfected and untreated control group); C-IF (infected control group); AB-IF (treated with IL-23^mAB and infected group); AB-C (treated with IL-23^mAB and uninfected group). (a) Semi-thin intestinal sections showing red (Alexa Fluor^® 594) signal for the labelled anti-CCL20 antibody. The cell nuclei were stained with DAPI (blue). (b) Measure of fluorescence intensity for CCL20 in four areas of 100 µm², mean ± SEM. (c) Semi-thin intestinal sections showing green (FITC) signal for anti-GRO antibody. The cell nuclei were labelled with DAPI (blue). (d) Measure of fluorescence intensity for GRO in four areas of 100 µm², mean ± SEM. The values are the means of the normalized expression values ± SEM. Tukey Test, p < 0.001 (***) and p < 0.05 (*).