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. 2018 May 17;8:7766. doi: 10.1038/s41598-018-25635-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of perlecan modifies the composition of myosin heavy chains by activating PGC1α. Detection by (a) SDS-PAGE-coupled silver staining and (b) relative composition of myosin heavy chain isoforms in the quadriceps of the WT-Tg and Hspg2^−/−-Tg mice. Soleus and plantaris represent markers for type I and II fibers, respectively. The relative intensity of the bands was quantified using ImageJ software. (c–e) Protein expression levels of (c) translocase of outer membrane 20 (TOM20), (d) translocase of inner membrane 23 (TIM23), and (e) peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) in the quadriceps of the WT-Tg and Hspg2^−/−-Tg mice. (f) Representative images of proteins extracted from quadriceps and stained with Ponceau S (Ponc) after SDS-PAGE. The relative intensities of the respective bands detected by western blotting using the specific antibody to the Ponc-stained patterns were quantified using ImageJ software. Data points and error bars represent the mean ± S.D. (n = 5 in a and b; n = 5–6 in c–e). Data were analyzed by two-way ANOVA with Sidak’s multiple comparison (b) and unpaired t-test (c–e). *p < 0.05, **p < 0.01, ***p < 0.001.