Table 2.

Combinations of genes from the xenovulene BGC used for heterologous expression experiments in A. oryzae

Exp.	Gene	aspks1	asL1	asL3	asR2	asL2	asL4	asL5	asL6	asR4	asR5	asR6	Metabolites
	annotation	PKS	FAD	NHI	P450	?	FAD	SDR	FAD	?	hDA	Hum
1		✓	–	–	–	–	–	–	–	–	–	–	4, 14
2		✓	✓	–	–	–	–	–	–	–	–	–	4, 14, 15
3		✓	✓	✓	–	–	–	–	–	–	–	–	4, 5, 14–16
4		✓	✓	✓	✓	–	–	–	–	–	–	–	4, 14–16,18,19, 21
5	Full	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	1, 4, 14–16,18–22
6	FullΔL5	✓	✓	✓	✓	✓	✓	–	✓	✓	✓	✓	1, 4, 14–16,18–22
7	FullΔR4	✓	✓	✓	✓	✓	✓	✓	✓	–	✓	✓	1, 4, 14–16,18–22
8	FullΔL2	✓	✓	✓	✓	–	✓	✓	✓	✓	✓	✓	1, 4, 14–16,18–22
9	Minimal	✓	✓	✓	✓	–	✓	–	✓	–	✓	✓	1, 4, 14–16,18–21, 22
10	FullΔR5	✓	✓	✓	✓	✓	✓	✓	✓	✓	–	✓	4, 14–16,18–21
11	FullΔR6	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	–	4, 14–16,18–21
12	FullΔL6	✓	✓	✓	✓	✓	✓	✓	–	✓	✓	✓	1a, 4, 7a, 14–16,18–21
13	FullΔL4	✓	✓	✓	✓	✓	–	✓	✓	✓	✓	✓	1a, 4, 8a, 14–16,18–21
14	MinimalΔL6L4	✓	✓	✓	✓	–	–	–	–	–	✓	✓	4, 12, 14, 15, 18, 21

Rows 1–4, genes homologous to TropA–D produce only polyketides; Rows 5–8, full cluster expression, and omission of superfluous genes (asl2, asL5 and asR4) produce 1. Rows 9–14, expression of minimal cluster and omission of genes encoding catalytically active proteins. Bold numbers indicate meroterpenoids. ^a = trace amounts