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. 2018 May 17;8:7753. doi: 10.1038/s41598-018-25964-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of POU5F1 CRISPR-induced mutation on bovine embryo development. (A) Embryo development following zygotic injection of CRISPR/Cas9 targeting the POU5F1 locus was determined at the 2C (cell), 8–16C and blastocyst stages (D7.5). (B) Representative brightfield image of D7.5 embryo morphology and blastocyst development in targeted knockout and injected controls. (C,D) Blastocyst development calculated based on the number of embryos reaching the 2C and 8–16 stage, respectively. ^a,bDifferent letters indicate significant differences (P < 0.05). Scale bar = 100 µm.