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. 2018 May 17;8:7753. doi: 10.1038/s41598-018-25964-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Embryonic POU5F1 is not required for D5 morula development. (A) Immunofluorescence staining for POU5F1 revealed embryos with negative, mosaic (MO) and positive expression in the CRISPR-targeted knockout group. (B) Quantification and characterization of POU5F1 expression patterns as negative (−/−), Mosaic or positive (+/+: >75% positive cells) from injected control and POU5F1 targeted embryos. * indicates difference (P < 0.05) between groups (TKO vs. IC) within mutation type. (C) Percentage of morulas with ≥16C that advanced from D3 to D5 in control and POU5F1 targeted groups. (D) Total cell number from injected control and targeted knockout (+/−, −/−) embryos. (E) Total cell number in targeted knockout embryos with zero (POU5F1^−/−) or mosaic (POU5F1^+/−) POU5F1 staining.