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. 2018 May 17;8:7753. doi: 10.1038/s41598-018-25964-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lack of POU5F1 does not alter SOX2 expression in D5 bovine morulas. (A) Temporal expression of SOX2 (red) and POU5F1 (green) in bovine embryos. (B) SOX2 (red) expression in POU5F1 (green) knockout and injected control D5 morulas. (C) Ratio of SOX2 positive cells to total cell number from injected control and POU5F1 targeted knockout groups (D). Number of SOX2 positive cells in POU5F1 positive and negative embryos within the targeted knockout group. Scale bar = 100 µm.