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Primer and sgRNA sequences (NCBI: AC_000180.1).
| Primers | POU5F1 | Sense | Length (nt) | |
|---|---|---|---|---|
| Primers (Outer): | Exon 1 | F | GTTGATCCTCGGACCTGGAT | 1077 |
| R | AGCGGGTTAGATGCAGAAGA | |||
| Exon 2 | F | CGTGTGTTTGTGAATGTGCG | 1242 | |
| R | GGAAAGAAATGGGCAGGCAA | |||
| Primers (Inner): | Exon 1 | F | CGCCCTATGACTTGTGTGG | 858 |
| R | CGAAATTGGTGTTCCAGCTT | |||
| Exon 2 | F | AGAGGGGGTGAGGTGGATAG | 854 | |
| R | CCAGTATCAGGGGGACAATG | |||
| sgRNA | Exon 1 | R | GCUUCUCCUUGUCCAGCUUC | |
| sgRNA | Exon 2 | F | GAUCACACUAGGAUAUACCC | |
