Fig. 5.

Production of partially reduced oxygen species at inactivated PS1 and free Chla under irradiation. Simultaneous photochronoamperometric responses recorded for a iPS1/Os-P and b free Chla/Os-P-modified electrodes. Electrolyte: 3 mM MV²⁺ in 0.2 M citrate-phosphate buffer, pH 7.0 (for b addition of MV²⁺ at 220 s). The sample was polarized at 0 mV, while the Pt disk microelectrodes were polarized at −600 mV (WE 1) and +600 mV (WE 2). All potentials vs. Ag/AgCl/3 M KCl. Tip-to-sample distance: 10 μm. The sample was locally irradiated (white light, 280 mW cm⁻²) for periods of 20 s, as indicated by the yellow shaded regions in the graphs