Table 1.
List of SUMO Sites Regulated by Senescence.
| Protein | Gene Name |
SUMO site | FCa | Uniqueb | Known PTMc |
|---|---|---|---|---|---|
| 78 kDa glucose-regulated protein | HSPA5 | 352 | 29.58 | x | Sm, Ac, Ub |
| Actin, cytoplasmic 1 | ACTB | 113 | 16.59 | x | Sm, Ac, Ub |
| Ribosome biogenesis protein BRX1 homolog | BRIX1 | 322 | 15.58 | x | Sm, Ub |
| NGFI-A-binding protein 1 | NAB1 | 480 | 8.86 | Sm | |
| Promyelocytic leukemia protein | PML | 490 | 8.53 | Sm, Ac | |
| Class E basic helix-loop-helix protein 40 | BHLHE40 | 279 | 8.00 | Sm | |
| Exosome component 10 | EXOSC10 | 583 | 6.74 | x | Sm |
| Unconventional myosin-Ib | MYO1B | 287 | 5.65 | Sm, Ub | |
| Small ubiquitin-related modifier 3 | SUMO3 | 41 | 3.72 | Sm, Ac, Ub | |
| Small ubiquitin-related modifier 2 | SUMO2 | 42 | 3.72 | Sm, Ac, Ub | |
| Nuclear autoantigen Sp-100 | SP100 | 297 | 3.57 | Sm | |
| SUMO-conjugating enzyme UBC9 | UBE2I/UBC9 | 49 | 3.36 | Sm, Ub | |
| MORC family CW-type zinc finger protein 3 | MORC3 | 740 | 3.13 | Sm | |
| Zinc finger MYM-type protein 4 | ZMYM4 | 250 | 0.74 | Sm | |
| Scaffold attachment factor B2 | SAFB2 | 230 | 0.74 | ||
| Putative oxidoreductase GLYR1 | GLYR1 | 176 | 0.57 | ||
| Transcription intermediary factor 1-beta | TRIM28 | 779 | 0.41 | Sm, Ac, Ub | |
| Transcription intermediary factor 1-beta | TRIM28 | 377 | 0.36 | Sm, Ac, Ub | |
| Histone deacetylase 1 | HDAC1 | 476 | 0.31 | Sm | |
| Symplekin | SYMPK | 483 | 0.24 | Sm | |
| Zinc finger protein 646 | ZNF646 | 1168 | 0.19 | Sm | |
| Cleavage stimulation factor subunit 2 | CSTF2 | 189 | 0.17 | Ub | |
| DNA repair protein complementing XP-C cells | XPC | 81 | 0.16 | Sm | |
| B-cell lymphoma/leukemia 11A | BCL11A | 799 | 0.15 | ||
| DNA repair protein complementing XP-C cells | XPC | 89 | 0.13 |
aFC: fold-change of SUMO site abundance in senescent cells divided by their abundance in controls cells, P-value < 0.05.
bUnique SUMO sites are defined as SUMO sites only observed in the senescent samples. FC are estimated using imputations of 1.8 standard deviations below the median for samples lacking a peptide abundance.
cKnown PTM reported at this site based on “PhosphoSite Plus” accessed on February 8, 2018.
Sm: SUMOylation; Ac: Acetylation; Ub: Ubiquitylation.
Gene names in bold correspond to proteins known to be associated with PML-NBs.