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. 2018 May 11;9:474. doi: 10.3389/fphar.2018.00474

FIGURE 1.

FIGURE 1

Competitive binding curves of 17β-estradiol (E2, 10 nM), (A) genistein (GEN; 10 nM), and (B) icariin (ICA; 0.1 μM) to ERα and ERβ. The binding of [3H]E2 to recombinant human ERα and ERβ was determined by subtracting the disintegration per minute (dpm) from the non-specific binding. The percentages of specific bound [3H]E2 were expressed as mean ± SEM (n = 5). Effects of treatment with (C) genistein and (D) icariin on ERα- or ERβ-mediated ERE luciferase activity in transfected UMR-106 cells. The transfected UMR-106 cells were treated with vehicle (C), 17β-estradiol (E2, 10-8 M), genistein (10-10–10-6 M), or icariin (10-10–10-6 M) for 24 h. Firely and Renilla luciferase activity was measured sequentially with Dual Luciferase Assay reagents. Results were expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus the control (n = 5).