FIGURE 1.

Electroconvulsive seizure (ECS)-induced hippocampal Bdnf expression is Egr3-dependent. Expression microarray analysis (A), and validation using qRT-PCR (B–D), of hippocampal Bdnf expression from WT, and Egr3–/–, mice at baseline (no ECS) and 1 h after ECS. Two-way ANOVAs showed significant interaction of genotype and ECS treatment on Bdnf expression in (A) male microarray data (p = 0.004) and qRT-PCR data in (C) female cohort 1 (p = 0.0005) and (D) female cohort 2 (p = 0.0003), and (B) significant effect of treatment in original male cohort (p = 0.0098; n = 4–5 animals/group; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001 controlled for multiple comparisons).