FIGURE 3.

Bdnf induction in the hippocampal dentate gyrus requires Egr3. Representative tissue sections demonstrating in situ hybridization labeling of Bdnf mRNA expressing cells in the hippocampus of WT and Egr3–/– mice at baseline (A,B) and 1 h following ECS (C,D). (A) Bdnf is expressed sparsely in CA3, CA2, and the DG of WT mice at baseline. (C) ECS induces high-level Bdnf expression in the DG, and increased expression in CA3, CA2, and CA1 of WT mice. (B) Bdnf expression in Egr3–/– mice is limited to rare cells in the CA3 region of the hippocampus, with no clear expression above background in the DG, CA2, or CA1, at baseline. (D) ECS produces little to no increase in Bdnf expression in the DG, CA3, CA2, and CA1 regions of Egr3–/– mice.