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. 2018 May 11;9:332. doi: 10.3389/fphys.2018.00332

Figure 3.

Figure 3

Increased susceptibility of RBCs from Nrf2 KO mice to t-BuOOH-induced impairment of deformability. (A) The ratio of GSH/GSSG in Nrf2 KO is increased as compared to WT mice (n = 5),*p < 0.05 student's t-test. (B) No difference exists between RBC deformability curves in wild type (WT) mice and Nrf2 KO mice as determined by ektacytometric analysis of RBC pellet two-way RM ANOVA p = 0.4497. (C) RBCs from Nrf2 KO mice show a significant decrease of EI values after treatment with 50 μM t-BuOOH to WT controls (n = 6). Two-way ANOVA p = 0.0341 and Sidak's test; *p ≤ 0.05, (D) RBCs from Nrf2 KO mice show a significant decrease of EI values after treatment with 100 μM t-BuOOH as compared to WT controls (n = 6). Two-way ANOVA p = 0.0095 and Sidak's test; ****p < 0.0001 over the whole range of shear stresses. All ektacytometric measurements were carried out from washed RBC diluted in PVP and measured in a range of shear stresses of 0.3–53.33 Pa.