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. 2018 May 11;9:332. doi: 10.3389/fphys.2018.00332

Figure 4.

Figure 4

NO does not influence RBC deformability measured by ektacytometry: (A) Treatment with the NO donor DEA/NO did not affect EI; the highest concentration (200 μM DEA/NO) significantly decreased RBC deformability. Two-way RM ANOVA p < 0.0001 and Dunnett's test vs. untreated control, *p ≤ 0.05 (statistically significant for 200 μM DEA/NO in the range of shear stresses between 0.3–5.3 and 30–53.33 Pa). (B) The Lineweaver–Burk transformation resulted in linear regression curves that are not significantly different from each other (n = 6). Two-way ANOVA p = 0.0015 and Dunnett's test vs. untreated control, ****p < 0.0001. (C) Deformability curves of RBCs from eNOS KO mice do not differ from that of WT mice. Two-way ANOVA p = 0.4725. (D) Lineweaver-Burk transformation of curves in C do not show any significant difference (n = 6). Two-way ANOVA p = 0.6029 All ektacytometric measurements were carried out from washed RBCs diluted in PVP solution and measured in a range of shear stresses of 0.3–53.33 Pa.