Table 4.

Total numbers and percentages of dual ChAT⁺/mRNA⁺ neurons in the PPN.

mRNA	mRNA+ neurons (n)	ChAT+ neurons (n)	Dually labeled neurons (n)	Percentage of ChAT+ neurons coexpressing mRNA
GAD65+	172.6 ± 44.4	174.4 ± 36.9	3.2 ± 1.6	1.96% ± 1.3
GAD67+	196.4 ± 47.7	151.6 ± 51.5	4 ± 2.2	2.91% ± 1.8
Vglut2+	232 ± 98.3	125.8 ± 26.3	10.2 ± 3.2	7.48% ± 1.8

Potential dually labeled cells were observed and counted during stereological quantification of single labeled ones in the PPN. As their low numbers precluded stereological estimations, we have calculated the relative percentages of these cells comparing directly the “within frame” counts of dual labeled cells with the concurrent “within frame” counts of single labeled ones. Data are expressed as mean ± SD, and were obtained from five animals.