Table 5.

Total numbers and percentages of dual ChAT⁺/mRNA⁺ neurons in the LDT.

mRNA	mRNA+ neurons (n)	ChAT+ neurons (n)	Dually labeled neurons (n)	% of ChAT+ neurons coexpressing mRNA
GAD65	301.2 ± 40.2	179.5 ± 13.9	5 ± 2.8	2.68% ± 1.3
GAD67	311.2 ± 1	157.6 ± 34.2	4.2 ± 2.4	2.46% ± 1
Vglut2	240.8 ± 34.4	132.2 ± 39.4	7.6 ± 1.8	5.1% ± 1.5

Potential dually labeled cells were observed and counted during stereological quantification of single labeled ones in the LDT. As their low numbers precluded stereological estimations, we have calculated the relative percentages of these cells comparing directly the “within frame” counts of dual labeled cells with the concurrent “within frame” counts of single labeled ones. Data are expressed as mean ± SD, and were obtained from five animals.