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. 2018 May 11;9:1031. doi: 10.3389/fimmu.2018.01031

Figure 1.

Figure 1

In vivo characterization of human memory natural killer (NK) cells. (A) Gating strategy for the identification of CD3−CD56dimCD16+FcεRIγ− memory NK cells. (B) Percentage of memory NK cells (with respect to total NK cells) in the peripheral blood of human cytomegalovirus (HCMV) seropositive (HCMV+) and seronegative (HCMV−) healthy donors; dotted line marks the value of 3%. (C) Percentage of total CD3−CD56+ NK cells among peripheral blood mononuclear leukocytes, in the same individuals. (D) Percentage of memory NK cells (with respect to total NK cells) in the peripheral blood of HCMV+ healthy donors, stratified according to CD16 allotype; dotted line marks the value of 3%. (E) Percentage of total CD3−CD56+ NK cells among peripheral blood mononuclear leukocytes, in the same individuals. CD16 mean fluorescence intensity (MFI) on memory (F) and total (G) NK cells of HCMV+ healthy donors, stratified according to CD16 allotype. Bars represent median and 10–90 percentiles. p values of pairwise comparisons are from Mann–Whitney non-parametric test.