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. 2018 May 11;9:1031. doi: 10.3389/fimmu.2018.01031

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Copyright © 2018 Capuano, Battella, Pighi, Franchitti, Turriziani, Morrone, Santoni, Galandrini and Palmieri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional responsiveness of fresh and in vitro cultured memory natural killer (NK) cells. Fresh (A) and cultured (B) memory (filled symbols) and conventional (empty symbols) NK cells were stimulated as indicated, and the percentage of CD107a/⁺IFN-γ⁺ was evaluated by immunostaining and FACS analysis. p values of pairwise comparisons are from Wilcoxon non-parametric test.