Fig. 5.

Incorporation of eGFP–MHC constructs into sarcomeres in cultured skeletal muscle myotubes formed by C2C12 cells. (a) Fluorescent images of skeletal muscle myotubes expressing eGFP–MHC constructs (green in merged image) co-stained for the Z-disc protein α-actinin (magenta in merged image). (b) Fluorescence images of skeletal muscle myotubes expressing GFP–MHC constructs (green in merged image) co-stained for the M-line protein myomesin (magenta in merged image). (c) Western blots for untransfected skeletal muscle myotubes (UT) and myotubes expressing WT and mutant eGFP–MHC constructs probed with anti-GFP. Gel lanes are approximately equally loaded, as shown by the blot probed with anti-α-tubulin (as a loading control).