Table III.

Secreted IFN-γ and TNF-α from T Cell Culture^*

Source	Cells	IFN-γ (pg/ml)	TNF-α (pg/ml)
Aged	PBMC	70,800 ± 8,500 (p < 0.001)	35,400 ± 6,200 (p < 0.005)
Control	PBMC	20,300 ± 3,000	16,900 ± 4,100
Aged	CD8+	86,300 ± 15,600 (p < 0.001)	44,200 ± 7,800 (p < 0.005)
Control	CD8+	24,400 ± 6,200	19,400 ± 4,300
HIV+	CD8+	121,000 ± 15,600 (p < 0.001)	57,200 ≅ 11,500(p < 0.001)
Control	CD8+	27,300 ± 4,800	16,400 ± 4,900

PBMC and purified CD8⁺ T cells were incubated for 66 hrs. as described in Methods, and the media was tested by ELISA. The results are expressed in pg IFN-γ or TNF-α per 10⁵ cells (mean value ± S.D.). The standard deviation is shown and the p valves, determined with Student's t test, compare the IFN-γ or TNF-α secreted by aged or HIV⁺-derived cells and normal control cells. Eight different aged and HIV⁺ donors were used, and seven young control donors. In the presence of 5 mM of N-acetylcysteine, the production of both INF-γ and TNF-α was significantly increased in aged, HIV⁺ and control samples, generally by 1.5 to 2 fold.