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Occurrence of ring-shaped nucleoli in MtTdp2α-overexpressing lines. (A) Ultrastructural morphology of a ring-shaped nucleolus (Tdp2α-13c line). n, nucleolus; np, nucleoplasm. (B) Distribution of ring-shaped nucleoli in 4-day old suspension cultures of CTRL, Tdp2α-13c, and Tdp2α-28 lines. Data represent the mean ± SD of three replicates per experiment. Asterisks indicate statistically significant differences determined using Student’s t-test (P < 0.05).
