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. 2018 May 11;9:596. doi: 10.3389/fpls.2018.00596

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Copyright © 2018 Macovei, Faè, Biggiogera, de Sousa Araújo, Carbonera and Balestrazzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of MtTdp2α gene confers tolerance to etoposide. MtTdp2α-overexpressing lines Tdp2α-13c and Tdp2α-28 and CTRL line exposed to increasing etoposide concentrations (0, 75, 150, and 300 μM) were analyzed using the DNA diffusion test at 0 and 6 h following treatments. The percentage of viable, PCD and necrotic cells is shown. For each treatment combination, data represent the mean values of three independent replications. Asterisks indicate statistically significant differences determined using Student’s t-test (P < 0.05).