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( a) Multiple sequence alignment highlighting the Pfcrt gene at the Apol 1 cutting region. ( b and c) Highlight point mutation in Pfcrt gene where the A nucleotide in a wild-type is substituted with C nucleotide in the mutant strain, ( d and e) the nucleotide sequence chromatogram of wild type and mutant strains respectively. ( f and g) The translated amino acid in wild and mutant strains, respectively.
