Transgenic mice with lineage-specific inhibition of NF-κB have reduced
RANKL expression in PDL fibroblasts not gingival fibroblasts.
(A) RANKL expression by fibroblastic cells in the PDL
was measured by immunofluorescence. (B) RANKL expression by
gingival fibroblasts was measured by immunofluorescence.
(C) RANKL mRNA levels were assessed by qRT-PCR when
stimulated with high glucose and/or TNF-α with or without the NF-κB
inhibitor BAY-117082. (D) RANKL protein levels in hPDLCs
were measured by ELISA assay when stimulated with high glucose and/or
TNF-α with or without BAY-117082 for 3 d. *P < 0.05 (vs. uninfected normoglycemic group).
**P < 0.05 (vs. matched wild-type
group). #P < 0.05 (vs. untreated
control group). ##P < 0.05 (vs. matched
control group). †P < 0.05 (vs. matched
normoglycemic group). hPDLC, human periodontal ligament cell; NF-κB,
nuclear factor kappa B; PDL, periodontal ligament; RANKL, receptor
activator of NF-κB ligand; qRT-PCR, real-time quantitative reverse
transcription polymerase chain reaction. Error bars indicate SEM.