Normoglycemic and diabetic transgenic mice with dominant negative
inhibition of NF-κB have reduced osteoclast numbers and alveolar bone
loss. Diabetes and periodontitis were induced in wild-type and
transgenic (Col1α1.IKKDN) mice by streptozotocin injection and oral
inoculation. All mice were euthanized 6 wk after completion of oral
bacterial inoculation. (A, D) TRAP analysis
were carried out to measure the osteoclasts (arrows) identified as
multinucleated cells harboring on the bone surface. (B,
E) Micro-CT analysis of remaining bone (the different
lengths of arrows indicate the difference in alveolar bone height)
between the molars in the mandible. (C, F)
Bone area was measured in H&E-stained sections between the molars in
the mandible. *P < 0.05 (vs. uninfected
normoglycemic group). **P < 0.05 (vs.
matched wild-type group). †P < 0.05
(vs. matched normoglycemic group). H&E, hematoxylin and eosin;
micro-CT, micro–computed tomography; NF-κB, nuclear factor kappa B;
TRAP, tartrate-resistant acid phosphatase. Error bars indicate SEM.