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Biotin-HA35 detection in the colon. Immunohistochemistry of colons removed from mice at the indicated hour time-point following a single 250 µl oral gavage of biotinylated HA35 (300 µg). Following extensive washing, biotin-HA35 was detected with streptavidin 700 (1:500 in PBS) and imaged by the Bruker In Vivo Xtreme system. Signal intensity measured as photons/sec/mm2. Abbreviations: HA35, 35 kDa hyaluronan; NEG, negative control.
