Figure 4.

HA35 inhibits Salmonella Typhimurium infection in vivo. The presence of Salmonella Typhimurium in intestinal and colon sections isolated from Salmonella infected mice treated orally with water (Panels A and C) or HA35 (300 µg daily; Panels B and D) was detected with rabbit anti-Salmonella antibody, followed by goat-anti-rabbit AlexaFluor 488 (green). Nuclei were stained with DAPI (blue). Panels A and B: terminal ileum; Panels C and D: proximal colon. Images were captured with a Leica upright fluorescent microscope and Image-Pro Plus software. Scale bar is 50 µm. Abbreviations: HA35, 35 kDa hyaluronan; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride.