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. 2018 Feb 27;9(5):137–150. doi: 10.1177/2042018818759894

Figure 3.

Figure 3.

Gene silencing of GPR132 does not affect the induction of FABP4 gene expression by HODEs. THP-1 differentiated macrophages were transfected with siGPR132 and the expression of GPR132 assessed by real-time PCR. GPR132 mRNA expression decreased 46% by siGPR132. Results are expressed as a percentage of control, mean ± SEM of triplicates from a representative experiment. (*p < 0.05, t test). B. THP-1 differentiated macrophages were treated with 9-HODE, 13-HODE or ROSI, and the gene expression of FABP4 assessed by real-time PCR. FABP4 expression was responsive to 9-HODE, 13-HODE and ROSI. The silencing of GPR132 (siGPR132) did not affect this induction. Results are expressed as a percentage of control, mean ± SEM [n = 3 separate experiments; *p < 0.01, two-way ANOVA, *p < 0.05, **p < 0.01 (compared against control, Tukey’s post-test corrected for multiple comparisons)].