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. 2018 Feb 27;9(5):137–150. doi: 10.1177/2042018818759894

Figure 4.

Figure 4.

Increased mRNA expression and protein secretion of FABP4 by THP-1 cells treated with HODEs is partially mediated by PPAR-γ. THP-1 cells were pre-treated with either the ROSI antagonist T0070907 or vehicle for 2 h, after which cells were washed and cultured in the presence of 9-HODE (30 µM), 13-HODE (30 µM) or ROSI (0.1 µM) for 24 h. (a) FABP4 mRNA levels were determined by real-time PCR. Treatment with 9-HODE, 13-HODE and ROSI induced an increase in FABP4 gene expression while pre-treatment with T0070907 significantly reduced this effect. (b) FABP4 protein concentration in tissue culture medium was measured by ELISA. Treatment with 9-HODE, 13-HODE and ROSI induced an increase in FABP4 gene expression while pre-treatment with T0070907 significantly reduced this effect. Results are expressed as mean ± SEM (n = 3 replicates per group). Groups with different superscripts are significantly different; p < 0.001; two-way ANOVA; Fisher’s multiple comparison test; representative data from three independent experiments.