Skip to main content
. Author manuscript; available in PMC: 2018 May 18.
Published in final edited form as: Cell Rep. 2018 Apr 24;23(4):1178–1191. doi: 10.1016/j.celrep.2018.03.118

Figure 1. miR-200s Positively Correlate with PKCζ in Colorectal Cancer.

Figure 1

(A) Venn diagram of common miRNAs identified from an miRNA microarray using SW480 cells, TCGA colon adenocarcinoma miRNA expression profile, and GSEA-C3 analysis of the mRNAs from the datasets.

(B–F) qPCR of miRNAs and immunoblot of PKCζ in SW480 shNT and shPKCζ cells, n = 4 biological replicates (B); SW480 and SW620 cells, n = 4 biological replicates (C); WT and PKCζ−/− mouse intestinal organoids, n = 3 biological replicates (D); ileum tissue 3 days post irradiation from Lgr5 and Lgr5;PKCζf/f mice, n = 3 mice/group (E); and intestinal tumor organoids from Lgr5;APCf/f and Lgr5;PKCζf/fAPCf/f mice, n = 3 biological replicates (F).

(G) Kaplan-Meier overall survival analysis of TCGA colon adenocarcinoma patients.

(H) Correlation between PRKCZ gene expression and the miR-200b/a/429 cluster in TCGA colon adenocarcinoma patients; n = 233 patients.

Results are presented as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001. See also Figure S1 and Table S1.