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. Author manuscript; available in PMC: 2018 May 18.
Published in final edited form as: Cell Rep. 2018 Apr 24;23(4):1178–1191. doi: 10.1016/j.celrep.2018.03.118

Figure 2. Loss of PKCζ Promotes EMT through miR-200s.

Figure 2

(A) qPCR of epithelial, mesenchymal, and stem-like genes in SW480 shNT and shPKCζ cells; n = 3 biological replicates.

(B) Immunoblot of EMT markers in SW480 shNT and shPKCζ cells; n = 3 biological replicates.

(C) Representative pictures of cell morphology of SW480 shNT and shPKCζ cells. Scale bar, 50 μm.

(D) GSEA plot of EMT, extracellular matrix remodeling, and stem-like signatures in low-versus high-PRKCZ-expressing tumors from TCGA-COAD. FDR, false discovery rate; NES, normalized enrichment score.

(E) Immunofluorescence for E-cadherin (left) and quantification of E-cadherin intensity (right) in SW480 shNT, shPKCζ, and shPKCζ cells rescued with miR-200a/b. Scale bar, 20 μm; n = 3 biological replicates.

(F) Immunofluorescence for Zeb1 (left) and quantification of Zeb1 intensity (right) in SW480 shNT, shPKCζ, and shPKCζ cells rescued with miR-200a/b. Scale bar, 20 μm; n = 3 biological replicates.

(G) qPCR of mesenchymal genes (left) and immunoblot of PKCζ (right) in SW480 shNT, shPKCζ, and shPKCζ cells rescued with miR-200a/b; n = 3 biological replicates.

(H) Representative pictures of cell morphology of SW480 shPKCζ cells rescued with miR-200a/b. Scale bar, 50 μm.

(I) Summary of the correlation between PRKCZ gene neighbors and CRC gene set signatures.

Results are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.