Table 2.

Percentages of predicted sequences assigned to tier 2 KO categories.

Functions∗	Fresh	Deep	Surface
Cell Processes†	2.05	1.79	1.74
Transport and Catabolism	0.18	0.19	0.18
Cell Growth and Death	0.39	0.42	0.42
Cell Motility	1.48	1.19	1.14
Environmental Information Processing	19.25	19.25	19.08
Membrane Transport	17.03	17.09	16.96
Signal Transduction	2.02	1.92	1.89
Signaling Molecules and Interaction	0.22	0.26	0.25
Genetic Information Processing	16.98	17.20	17.34
Transcription	3.03	3.02	3.00
Translation	4.60	4.75	4.81
Folding, Sorting and Degradation	2.02	1.91	1.94
Replication and Repair	7.35	7.54	7.60
Human Diseases	0.87	0.85	0.87
Cancers	0.06	0.04	0.04
Immune System Diseases	0.06	0.06	0.06
Infectious Diseases	0.51	0.50	0.51
Metabolic Diseases	0.08	0.08	0.08
Neurodegenerative Diseases	0.17	0.18	0.18
Metabolism	45.03	45.70	45.57
Amino Acid Metabolism	8.28	8.15	8.14
Biosynthesis of Other Secondary Metabolites	0.73	0.73	0.72
Carbohydrate Metabolism	11.57	12.24	12.11
Energy Metabolism	4.64	4.53	4.55
Enzyme Families	1.97	1.90	1.90
Glycan Biosynthesis and Metabolism	1.85	1.61	1.71
Lipid Metabolism	2.98	3.11	3.06
Metabolism of Cofactors and Vitamins	3.54	3.29	3.37
Metabolism of Other Amino Acids	1.61	1.60	1.61
Metabolism of Terpenoids and Polyketides	1.65	1.79	1.75
Nucleotide Metabolism	3.75	3.97	3.98
Xenobiotics Biodegradation and Metabolism	2.52	2.83	2.71
Organismal Systems	0.41	0.35	0.36
Digestive System	0.03	0.02	0.02
Endocrine System	0.16	0.15	0.15
Excretory System	0.02	0.01	0.01
Immune System	0.04	0.03	0.03
Nervous System	0.05	0.05	0.05
Environmental Adaptation	0.10	0.09	0.09

^∗Functional categories for which no reads were assigned are omitted. ^†Bolded functions represent tier 1 functional categories.