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. 2018 Apr 16;15(6):9081–9086. doi: 10.3892/ol.2018.8507

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Copyright: © Fei et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — GPC3 reverses partial fuction of miR-96 on proliferation. (A) The expression of GPC3 was detected by RT-qPCR when transfected siRNA-GPC3 or siRNA-NC into A549 cells. (B) The proliferative ability was measured after A549 cells were transfected with siRNA or negative control. (C) The cells transfected with both miR-96 mimic and pcDNA3.1-GPC3 exhibited a lower GPC3 mRNA level compared with the cells transfected with pcDNA3.1-GPC3. (D) The proliferative ability was measured after A549 cells were co-transfected with miR-96 mimic and pcDNA3.1-GPC3 or only transfected with pcDNA3.1-GPC3. GPC3, glypican-3; RT-qPCR, reverse transcription-quantitative PCR. *P<0.05; ***P<0.001.